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ceramic hydroxyapatite column  (Bio-Rad)


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    Structured Review

    Bio-Rad ceramic hydroxyapatite column
    Ceramic Hydroxyapatite Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ceramic+hydroxyapatite+cartridge/Bio-Scale+Mini+CHT+Type+II+Cartridge/10__1016_slash_j__pmpp__2025__103028-109-20-29
    Average 93 stars, based on 24 article reviews
    ceramic hydroxyapatite column - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Structural and Functional Studies of the Membrane Binding Domain of NADPH -Cytochrome P450 Oxidoreductase
    Article Snippet: Excess spin label was removed by loading the solution onto a Bio-Rad Mini CHT-I Ceramic Hydroxyapatite Cartridge followed by extensive washing with 10 mM KPi pH 7.4, containing 10% glycerol.

    Article Title: YdiV: a dual function protein that targets FlhDC for ClpXP-dependent degradation by promoting release of DNA-bound FlhDC complex
    Article Snippet: The fractions containing ClpX were loaded onto Bio-ScaleTM Mini CHTTM Type I Ceramic hydroxyapatite cartridge (5 ml, Bio-Rad) and then washed with buffer D containing 2 mM ATP.

    Article Title: YdiV: a dual function protein that targets FlhDC for ClpXP-dependent degradation by promoting release of DNA-bound FlhDC complex
    Article Snippet: The fractions containing ClpP were loaded onto Bio-ScaleTM Mini CHTTM Type I Ceramic hydroxyapatite cartridge (5 ml, Bio-Rad) and then washed with buffer D (50 mM Tris-HCl, pH 7.2, 5 mM MgCl 2 , 5 mM DTT, 10% glycerol).


    Purification:

    Article Title: Granzyme A Stimulates pDCs to Promote Adaptive Immunity via Induction of Type I IFN
    Article Snippet: .. The GzmA was further purified using a Bio-ScaleTM Mini CHTTM Type I ceramic hydroxyapatite cartridge (Bio-Rad) equilibrated with 2xPBS buffer and was eluted with an NaCl (0-1M linear gradient) in 3x PBS. .. Purified GzmA was diluted 3- fold with distilled water and concentrated by ultrafiltration with an Amicon Ultra-15 30K-MWCO filter (Millipore).



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    Purification profile of natural ( A ) and recombinant ( B ) tropomyosin of Pacific oyster. Natural tropomyosin was purified using <t>CHT™</t> Ceramic <t>Hydroxyapatite.</t> The increase in the absorbance was measured at 280 nm (blue line) and 220 nm (red line) and 31 eluted fractions were collected and analysed by SDS-PAGE. The 17th, 18th, and 19th peak contained pure tropomyosin, appearing as a strong band at 39 kDa in the Coomassie-stained SDS-PAGE gel. Recombinant tropomyosin (3B) was purified using HisPur Ni-NTA with increasing concentration of imidazole. Purified tropomyosin was observed at 40 kDa due to six-His-tag fused to its N-terminal. Note for B: CE = crude extract, FT = Flow through, W = Wash, E = Eluent.
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    Purification profile of natural ( A ) and recombinant ( B ) tropomyosin of Pacific oyster. Natural tropomyosin was purified using <t>CHT™</t> Ceramic <t>Hydroxyapatite.</t> The increase in the absorbance was measured at 280 nm (blue line) and 220 nm (red line) and 31 eluted fractions were collected and analysed by SDS-PAGE. The 17th, 18th, and 19th peak contained pure tropomyosin, appearing as a strong band at 39 kDa in the Coomassie-stained SDS-PAGE gel. Recombinant tropomyosin (3B) was purified using HisPur Ni-NTA with increasing concentration of imidazole. Purified tropomyosin was observed at 40 kDa due to six-His-tag fused to its N-terminal. Note for B: CE = crude extract, FT = Flow through, W = Wash, E = Eluent.
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    Purification profile of natural ( A ) and recombinant ( B ) tropomyosin of Pacific oyster. Natural tropomyosin was purified using CHT™ Ceramic Hydroxyapatite. The increase in the absorbance was measured at 280 nm (blue line) and 220 nm (red line) and 31 eluted fractions were collected and analysed by SDS-PAGE. The 17th, 18th, and 19th peak contained pure tropomyosin, appearing as a strong band at 39 kDa in the Coomassie-stained SDS-PAGE gel. Recombinant tropomyosin (3B) was purified using HisPur Ni-NTA with increasing concentration of imidazole. Purified tropomyosin was observed at 40 kDa due to six-His-tag fused to its N-terminal. Note for B: CE = crude extract, FT = Flow through, W = Wash, E = Eluent.

    Journal: Foods

    Article Title: Recombinant Tropomyosin from the Pacific Oyster ( Crassostrea gigas ) for Better Diagnosis

    doi: 10.3390/foods11030404

    Figure Lengend Snippet: Purification profile of natural ( A ) and recombinant ( B ) tropomyosin of Pacific oyster. Natural tropomyosin was purified using CHT™ Ceramic Hydroxyapatite. The increase in the absorbance was measured at 280 nm (blue line) and 220 nm (red line) and 31 eluted fractions were collected and analysed by SDS-PAGE. The 17th, 18th, and 19th peak contained pure tropomyosin, appearing as a strong band at 39 kDa in the Coomassie-stained SDS-PAGE gel. Recombinant tropomyosin (3B) was purified using HisPur Ni-NTA with increasing concentration of imidazole. Purified tropomyosin was observed at 40 kDa due to six-His-tag fused to its N-terminal. Note for B: CE = crude extract, FT = Flow through, W = Wash, E = Eluent.

    Article Snippet: Hence, 20 mg of protein was diluted in buffer containing 5 mM NaPO 4 , 150 mM NaCl, pH 6.8, and loaded onto a Bio-scale Mini CHT Ceramic Hydroxyapatite Cartridges column (BioRad, Hercules, CA, USA).

    Techniques: Purification, Recombinant, SDS Page, Staining, Concentration Assay